生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (2): 52-.doi: 10.3969/j.issn. 2095-1736.2022.02.052

• 研究报告 • 上一篇    下一篇

大熊猫尿液成分的代谢组学研究

  

  1. 1. 四川农业大学 资源学院， 成都 611130； 2. 大熊猫国家公园珍稀动物保护生物学国家林业和
    草原局重点实验室（中国大熊猫保护研究中心）， 都江堰 611830
  • 出版日期:2022-04-18 发布日期:2022-04-18
  • 通讯作者: 邹立扣，教授，博士生导师，主要从事资源微生物及耐药性等研究，E-mail: zoulikou@sicau.edu.cn
  • 作者简介:谢婷霞，硕士研究生，专业方向为资源利用与植物保护，E-mail：xietingxia0615@163.com；李才武，硕士，工程师，主要从事大熊猫兽医研究，E-mail:yaomao712@sina.com；谢婷霞和李才武为共同第一作者
  • 基金资助:
    大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室开放基金项目（中国大熊猫保护研究中心，KLSFGAGP2020.003）;大熊猫国际合作基金项目（2017-115）

Urine metabolomics of giant panda (Ailuropoda melanoleuca)

  1. 1. College of Resources, Sichuan Agricultural University, Chengdu 611130, China；
    2. Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of
    Rare Animals in the Giant Panda National Park, China Conservation and Research Center for
    the Giant Panda, Dujiangyan 611830, China
  • Online:2022-04-18 Published:2022-04-18

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摘要： 为探究大熊猫（Ailuropoda melanoleuca）尿液代谢物的差异性，采用液相色谱-质谱UPLC-Q-TOF/MS技术对20例大熊猫尿液代谢成分进行检测，并通过性别和年龄分组对其尿液代谢物进行差异分析。研究发现，在性别组间共鉴定出12种显著差异代谢物（P＜0.05），其中，除芹菜素外其余11种代谢物的相对含量均在雄性尿液中更高。亚成年组与成年组间共鉴定出25种显著差异代谢物（P＜0.05），仅核黄素和精氨酸的相对含量在亚成年个体尿液中更高；而亚成年与老年组间和成年组与老年组间分别共鉴定出10种和3种显著差异代谢物（P＜0.05），其相对含量均在老年个体尿液中更高。这些显著性差异代谢物主要集中在氨基酸代谢、次生代谢物的生物合成及黄酮、黄酮醇和类黄酮的生物合成通路。对不同性别、年龄间大熊猫尿液代谢物进行差异性分析，筛选出的差异代谢物为大熊猫化学通讯机理和生理功能的研究提供理论基础。

关键词: 大熊猫, 差异代谢物, 代谢通路, 生物合成通路, 代谢组学, 化学通讯

Abstract: In order to explore the difference of metabolites in giant panda urine, 20 cases of giant panda urine metabolites were detected by liquid chromatography-mass spectrometry (UPLC-Q-TOF/MS), and differences in urine metabolites were analyzed according to gender and age. As results, 12 metabolites with significant difference （P＜0.05）were identified between the gender group, and the relative expression of 11 metabolites except for apigenin was higher in male urine. A total of 25 significantly different metabolites （P＜0.05） were identified between the sub-adult group and the adult group. Only the relative expression levels of riboflavin and arginine were higher in the urine of the sub-adult giant panda. Ten significantly different metabolites （P＜0.05）were identified between the sub-adult group and the geratic group, and three significantly different metabolites were identified between the adult group and the geratic group. The relative expression of these substances was higher in the urine of elderly individuals. These metabolites with significant differences were mainly concentrated in the amino acid metabolism pathway, the biosynthesis pathway of secondary metabolites and the biosynthesis pathway of flavones, flavonols and flavonoids. The urine metabolites of giant pandas of different genders and ages were analyzed in this study.The differential metabolites obtained by screening can provide a theoretical basis for the study of chemical communication mechanism and physiological function of giant pandas.

Key words: giant panda, differential metabolites, metabolic pathway, biosynthetic pathway； metabonomics； chemical communication

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